Fig. 1.

a Map of pNEOL102 containing the maqRt and G418Rt cassettes. Black arrows show the position of oligonucleotides O21+ and O22− used for PCR analysis, and oligonucleotides O66+ and O66− used for hybridization analysis (785 bp probe). LB T-DNA left border; RB T-DNA right border. b Amplification of maqRt cassette from genomic DNA. M molecular weight marker III; 1 R. toruloides CECT13085 clone #24; 2 R. toruloides CECT13085 NS-134 strain; 3 pNEOL102; 4 R. toruloides CECT13085 wild-type strain and c hybridization analysis. M, molecular weight marker III DIG-labeled; 1 R. toruloides CECT13085 wild-type strain; 2 clone #24; 3 NS-134 strain