Figure 1.

Hybrid networks generated by MetaCore analysis of proteins differentially expressed in bone and skin of lethal and surviving mutant mice and WT littermates. (A) Bone/skin hybrid network. Proteins detected as differentially expressed in skin, and experimentally proven to be deregulated also in bone by western blot assays, are highlighted by continuous red line. Proteins that were found differentially expressed both in skin and bone are circled in dashed red. (B) Skin/bone hybrid network. Proteins that were found differentially expressed in bone are surrounded by continuous green line, whereas proteins observed differentially expressed both in skin and bone are circled in dashed green. Network proteins are visualized by specific symbols, which define the functional nature of the protein (network caption). The edges indicate the relationships existing between individual proteins, and the arrowheads represent the direction of the interaction. The following line colors designate the nature of the interaction: red: negative effect; green: positive effect; gray: unspecified effect. PSMC6: 26S protease regulatory subunit S10B; PSMB3: proteasome subunit beta type-3; BMP: bone morphogenic protein.