Expression analysis of some focal hubs from hybrid nets in skin, bone and lung tissues. (A) Representative western blots of stathmin and corresponding densitometric analysis are shown. In all the three analyzed tissues, stathmin is significantly up-regulated in Brtl+/− lethal (ML) mice with respect to surviving Brtl+/− mice (MA) and WT littermates. (B) Representative western blots of cofilin-1 and corresponding densitometric analysis are shown. In all the three analyzed tissues, cofilin-1 is significantly up-regulated in Brtl+/− ML mice. Histograms visualize normalized mean relative-integrated density ± SD values. *, ¥ and § symbols indicate the significance of expression changes occurring between WT and ML, WT and MA, and ML and MA mice, respectively.