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. 2015 May 7;146(2):265–280. doi: 10.1093/toxsci/kfv091

FIG. 4.

FIG. 4.

HepG2 cells were treated with (A) a representative AA derivative (DCLF: 250  µM) or (B) a representative PA derivative (IBU: 6 mM) alone or in combination with TNFα and/or IFNγ, and protein extracts were collected 12 or 18 hours after treatment. p-JNK and total JNK protein were detected via western analysis. Representive blots are shown. Densitometry was performed using image J software. a, significantly different from VEH within an NSAID treatment. b, significantly different from IFNγ within an NSAID treatment. c, significantly different from Control within a cytokine treatment. d, significantly different from TNFα within an NSAID treatment. Data are represented as mean ± S.E.M of 3 separate experiments. Abbreviations: VEH, vehicle; TNF, tumor necrosis factor-alpha; IFN, interferon gamma; TI, tumor necrosis factor-alpha and interferon-gamma; DCLF, diclofenac; IBU, ibuprofen.