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. 2015 Oct;185(10):2757–2767. doi: 10.1016/j.ajpath.2015.06.006

Figure 2.

Figure 2

TMIGD1 promotes TEER of epithelial cells. A: Fully confluent HEK-293 cells that expressed TMIGD1 or control empty vector were seeded on the collagen-coated trans-wells, and electrical resistance was measured at day 0 and 5. B: Fully confluent HEK-293 cells expressing TMIGD1 or control vector were seeded on the collagen-coated trans-wells and subjected to permeability assay with fluorescently labeled dextran. Permeability was measured at 30, 60, and 120 minutes. C: HK2 cells that expressed control shRNA or TMIGD1 shRNA were seeded on the collagen-coated trans-wells, and electrical resistance was measured as A. The silencing effect of TMIGD1 shRNA on the expression of TMIGD1 in HK2 cells is shown. Whole cell lysates was blotted for TMIGD1 and loading control, Hsp70. D: HEK-293 cells that expressed TMIGD1 or empty vector were stained for actin with the use of phalloidin. Shown is the typical structural characteristics of HEK-293 cells that expressed TMIGD1 or control vector. Photographs were taken under light microscope. n = 4 Transwell per group; n = 2 independent experiments (A–C). Ctr, control; Hsp70, heat shock protein 70; pMSCV, empty retroviral vector; TEER, transepithelial electrical resistance; TMIGD1, transmembrane and immunoglobulin-containing 1.