Supplemental Figure S1.

Expression of Galgt2 protein and CT glycan in developing and regenerating skeletal muscle and in embryonal rhabdomyosarcoma. CT2 staining of CT glycan and Galgt2 immunostaining increase in day 4 regenerating muscle after cardiotoxin injection (P4 Ctx) and in embryonal rhabdomyosarcoma (eRMS). Developing normal muscle shows high CT glycan expression (stained with CT2) at postnatal day P1, with primarily synaptic concentration by P14 and P42. P42 staining serves as a primary antibody control for P4 Ctx staining in a normal muscle. Control shows staining with secondary antibody alone. Scale bar = 100 μm.