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. 2015 Oct;185(10):2790–2804. doi: 10.1016/j.ajpath.2015.06.007

Figure 3.

Figure 3

iNOS interacts with NHERF2 in UC-affected colon tissues. A: Immunohistochemical staining for iNOS and NHERF2 expression in non-UC and UC-affected human colon tissue sections with the use of DAB-based method. Arrows indicate membrane localization of iNOS and NHERF2. B: Representative images show the interaction between iNOS and NHERF2 in human colon biopsies in PLA. Strong PLA signals (red; marked arrows) are observed in UC-affected human colon tissue section, showing the interaction between iNOS and NHERF2, whereas considerably low PLA signals are observed in non-UC colon. Scale bar = 100 μm. DAB, 3-3′-diaminobenzidine; iNOS, inducible nitric oxide synthase; NHERF2, Na+/H+ exchanger regulatory factor 2; PLA, proximity ligation assay; UC, ulcerative colitis.