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Western blot autoradiographs and calibration plots. Western blot autoradiographs for single standard sample of the mixture of all Y. pestis strains protein extracts containing 15, 30, 45, and 60 μg of protein, respectively. Calibration plots are shown below each autoradiograph and reflect the linear response of immunostaining with the corresponding amount of loaded protein for (A) F1 antigen, (B) outer membrane protein C, porin, (C) Tellurium, and (D) DNA-binding protein H-NS.
