FIGURE 4.

Neutropenic mice show high bacterial burden in superficial skin lesions. (A) Wound bacterial burden (CFU; colony forming units) in control IgG2a and mAb 1A8-treated mice infected with 10⁷ A. baumannii CFU. Three and seven days post-infection, infected skin tissue was removed from mice and bacterial burden determined. Bars are the averages of the results for seven clinical isolates (each symbol represent a strain; n = 7), and error bars denote standard deviations. Asterisks denote P-value significance (^∗P < 0.05) calculated by student’s t-test analysis. This experiment was performed twice with similar results obtained. (B) Histological analysis of Balb/c mice infected with A. baumannii 0057, days 3 and 7. Representative 20X (upper panel) and 40X (lower panel; magnified black boxes in upper panel) H&E-stained sections of the skin lesions are shown with the insets representing Gram staining for A. baumannii cells (shown in pink-red spots; lower panel). Scale bars: 20 μm.