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. 2015 Oct 16;6:1134. doi: 10.3389/fmicb.2015.01134

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Copyright © 2015 Grguric-Smith, Lee, Gandhi, Brennan, DeLeon-Rodriguez, Coelho, Han and Martinez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutropenia altered pro-inflammatory cytokine production. Homogenates of extracted wounded tissue from control IgG2a + A. baumannii and 1A8 + A. baumannii mice, 3 and 7 days post-infection were prepared and the supernatants were analyzed for (A) TNF-α, (B) IFN-γ, (C) IL-1β, and (D) IL-6 levels. Bars represent the mean values for seven clinical isolates (each symbol); error bars denote standard deviations. For (A–D), asterisks denote P-value significance (^∗P < 0.05) calculated using student’s t-test analysis. Cytokine quantification was performed thrice with similar results obtained.