Table 1. DNA damage and its suppression during Cd-induced malignant transformation of 16HBE cells and siRNA/ENST00000414355 transfected 35th passage cells determined by comet assay.
Cell type | DNA damage rate (%) | Tail length (μm) |
---|---|---|
Untransformed 16HBE cells | 4.75 | 12.8 ± 1.76 |
5th passage | 10.45# | 15.2 ± 3.54# |
15th passage | 22.00*# | 31.6 ± 2.80*# |
35th passage | 46.75* | 47.8 ± 2.36* |
siRNA/ENST00000414355-4311 transfected 35th passage cells | 24.79*# | 33.8 ± 4.43*# |
siRNA/ENST00000414355-4312 transfected 35th passage cells | 23.98*# | 32.4 ± 4.17*# |
Negative control transfected 35th passage cells | 41.14* | 44.56 ± 5.17* |
The tail lengths of cells in which DNA damage was induced and suppressed were determined by comet assay in untransformed 16HBE cells, 5th, 15th, 35th passage transformed cells, siRNA/ENST00000414355 transfected 35th passage cells and Negative control transfected 35th passage cells. *P<0.05, vs untransformed controls, #P<0.05, vs 35th passage cells.