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. 2015 Oct 16;5:15321. doi: 10.1038/srep15321

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Flow cytometer gating strategy for sperm treated with seawater (control: (a,b)) and egg water (seawater + egg water, 1:1: (c,d)). Gating was based on FSC and SSC area of the sperm, which separated sperm from the other particles of the seawater (e) and egg water (f). The sub-population “Non-reacted” represents sperm with intact acrosomes, while the sub-population “AR” represents acrosome-reacted sperm. Numbers indicate proportions (%) of gated events of all events.