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. 2015 Oct 16;5:15409. doi: 10.1038/srep15409

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Phylogenetic distance was calculated with combined NBD-TMD amino acid sequences from O. melastigma and other species. A best-fit substitution model was established using maximum likelihood (ML) analysis. Numbers at nodes represent the ML bootstrap support values and Bayesian posterior probabilities (=1.00). Details on the model test and parameters are explained in the Materials and Methods section. The tree is proportionally scaled and the scale bar indicates sequence distance in units of substitutions. Species abbreviations: Dr, Danio rerio; Hs, Homo sapiens; Ip, Ictalurus punctatus; Mm, Mus musculus; Ol, Oryzias latipes; Om, Oryzias melastigma; On, Oreochromis niloticus; Tn, Tetraodon nigroviridis.