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. 2015 Oct 16;5:15409. doi: 10.1038/srep15409

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Phylogenetic distance was calculated with combined NBD-TMD amino acid sequences from O. melastigma and other species. A best-fit substitution model was established using maximum likelihood (ML) analysis. Numbers at nodes represent the ML bootstrap support values and Bayesian posterior probabilities. The tree is proportionally scaled, with the scale bar indicating sequence distance in units of substitutions. Species abbreviations: Am, Astyanax mexicanus; Dr, Danio rerio; Ga, Gasterosteus aculeatus; Hb, Haplochromis burtoni; Hs, Homo sapiens; Ip, Ictalurus punctatus; Lo, Lepisosteus oculatus; Mm, Mus musculus; Mz, Maylandia zebra; Nb, Neolamprologus brichardi; Ol, Oryzias latipes; Om, Oryzias melastigma; On, Oreochromis niloticus; Pf, Poecilia Formosa; Pn, Pundamilia nyererei; Tn, Tetraodon nigroviridis; Tr, Takifugu rubripes.