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. 2015 Oct 16;5:15221. doi: 10.1038/srep15221

Figure 8. VEGF and its receptor decrease during PGN+poly(I:C)-induced preterm labor and inhibition of Notch signaling suppresses VEGF secretion.

Figure 8

(A) The mRNA expression of VEGF in placenta recovered from PBS and PGN+poly(I:C) treated groups. N = 6–11 each group. *P ≤ 0.05 Significant difference vs. PBS (B) Protein concentration of VEGF measured by Luminex assay in protein extracted from placental cells recovered from mouse on day 14.5 of pregnancy, cultured ex vivo and treated with PBS and PGN+poly(I:C) for 2 h, followed by treatment with either control or GSI for 10 h. N = 3 each group. *P ≤ 0.05 Significant difference between PGN+poly(I:C) treated with control/GSI. (C) Immunofluorescence staining (Upper panel) and mean integrated density value (IDV) (lower panel) of VEGF-R (green) in placenta. Nuclei stained with DAPI (blue) in merged images. N = 4-5 each group. Six sections per animal were analyzed. Original magnification: 200 X. Bars: 10 μm. PBS and PGN+poly(I:C): intrauterine injections on day 14.5. Error bars = ±SEM. **P ≤ 0.01 Significant difference vs. PBS.