Figure 13. The validation of exonic structure and differential expressions of candidate lincRNAs.
(A) The validation of lincRNA structure using ordinary PCR. Lane S1 and S2: amplified fragments for target lincRNAs with dscDNA as the template and corresponding lincRNAs were indicated on the gel; Lane Geno: amplified fragments for specific lincRNAs with chicken genomic DNA as the template. (B) The validation of differentially expressed lincRNAs using qPCR assay. Different comparisons were labeled on the graph. LincRNA loci with more than 2-fold changes are considered differentially expressed and are marked by a red star. Also, the differentially expressed lincRNAs were labelled with p-value.