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. 2015 Oct 12;6:8440. doi: 10.1038/ncomms9440

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(a) Minicircle DNA incubated with nuclease Bal-31. Over time, samples were removed, quenched by the addition of stop buffer and the products analysed by polyacrylamide gel electrophoresis. Mr: 100 bp DNA ladder, L: linearized 336 bp DNA, N: nicked 336 bp minicircle. (b) Graphic representation of the data shown in (a) Fitted lines are for visualization purposes only. (c) MD simulation of the ΔLk=−3 topoisomer in explicit solvent. Splayed bases were found at a sharp bend of a needle conformation. This may be a potential atomistic explanation for Bal-31 susceptibility of negatively supercoiled topoisomers.