Extended Data Figure 2. Resolution estimation and example of de novo model building.

(a) Gold standard Fourier Shell Correlation (FSC) curve and FSC curve between cryo-EM map and final atomic model of the APC/C^Cdh1.Emi1. (b) Cross-validation of model refinement by half maps. Shown are FSC curves between the atomic model and the half map (map^half1) it was refined against and FSC curve between the atomic model and the other half map (map^half2) that was not used during refinement. (c) Gold standard FSC curve of APC/C^{Cdh1.Hsl1.UbcH10-Ub}. (d) Gold standard FSC curves of human APC/C^{Cdh1.Hsl1.Apc11-UbcH10}. (e) de novo model building of Apc15 N-terminal loop. The surrounding Apc5 residues are also shown. (f) EM density for the LR tail common to Emi1 and Ube2S is only observed in APC/C complexes with subunits incorporating the LR tail. (i) APC/C^Cdh1.Emi1. (ii) An APC/C complex with an LR tail-bearing subunit (UbcH10^LR of APC/C^{Cdh1.Hsl1.UbcH10-Ub}). (iii) No LR tail density in APC/C^{Cdh1.Hsl1.Apc11-UbcH10} fusion and (iv) APC/C^Cdh1.Hsl1 (Ref. ⁴).