. Author manuscript; available in PMC: 2015 Dec 25.

Published in final edited form as: Nature. 2015 Jun 15;522(7557):450–454. doi: 10.1038/nature14471

Extended Data Table 3.

a. Inter-subunit contact surface area Å².
Subunit	Apc1	Apc2	Apc3	Apc4	Apc5	Apc6	Apc7	Apc8	Apc10	Apc11	Apc12	Apc13	Apc15	Apc16	Cdh1	Emi1
Apc1	-
Apc2	2520	-
Apc3	360	0	5746
Apc4	0	714	0	-
Apc5	8744	0	0	6226	-
Apc6	1056	0	4590	0	0	5244
Apc7	0	0	5354	0	0	0	5026
Apc8	4290	0	0	0	3722	3772	0	4558
Apc10	2616	0	2510	0	0	0	0	0	-
Apc11	0	3402	0	0	0	0	0	0	0	-
Apc11^RING	0	666	0	0	0	0	0	0	0	0
Apc12	246	0	0	0	0	5970	0	0	0	0	-
Apc13	0	0	836	0	0	2068	0	3240	0	0	0	-
Apc15	0	0	0	224	3446	340	0	1082	0	0	0	0	-
Apc16	40	0	2514	0	0	222	1502	0	0	0	0	0	0	-
Cdh1	1960	0	1906	0	0	712	0	3022	0	0	0	0	0	0	-
Cdh1^NTD	1960	0	0	0	0	500	0	3022	0	0	0	0	0	0	0	0
Cdh1^WD40	0	0	82	0	0	212	0	0	0	0	0	0	0	0	0	0
Cdh1IR	0	0	1824	0	0	0	0	0	0	0	0	0	0	0	0	0
Emi1	732	762	0	0	0	0	0	0	704	1300	0	0	0	0	754	-
Total	24524	8064	25722	7164	34010	24686	11882	26708	5830	4702	6216	6144	5092	4278	8354	4252

b. DALI Results
Subunit/domain	Protein match	RCSB PDB	Z	RMSD Å	Aligned N	Res N	ID %	Subunit/domain	Protein match	RCSB PDB	Z	RMSD Å	Aligned N	Res N	ID %
Apc1^WD40	Sc Cdh1	4BH6-E	15.9	4.0	259	303	8	Apc6	Sp Apc6	2XPI-D	36.9	2.2	481	520	37
Apc1^PC	Sc Rpn2	4ADY-B	27.8	3.2	406	802	15	Apc7	Sp Apc6	2XPI-D	24.9	7.8	464	518	15
Apc2^NTD	Hs Cul3	4HXI-B	13.8	7.4	301	346	7	Apc8	Sp Apc6	2XPI-D	25.5	7.2	443	518	19
Apc2^CTD	Hs Cullin1	4F52-A	23.5	2.2	237	258	18	Apc3A	Apc3B	-		1.6	482	497	100
Apc5^TPR	Sp Apc6	2XPI-A						Apc6A	Apc6B	-		4.0	486	503	100
Apc3	Sp Apc6	2XPI-A	29.6	4.9	456	518	19	Apc7A	Apc7B	-		2.6	483	483	100
								Apc8A	Apc8B	-		3.7	389	490	100

c. Surface Plasma Resonance Data
Ligand	Analyte	k_off (s⁻¹)	k_on (M⁻¹ s⁻¹)	K^eq_d (nM)	K^kin _d (nM)	Reference
UbcH10	Ternary	0.42 ± 0.02	1.78 ± 0.2 × 10⁶	215	236 ± 29	4
UbcH10^LR	Ternary (fast)	4.0 ± 0.2 × 10⁻²	1.70 ± 0.1 × 10⁶	31	24 ± 2	This study
UbcH10^LR	(slow)	4.6 ± 0.2 × 10⁻³	1.70 ± 0.1 × 10⁵	31	23 ± 5	This study

K^kin_d is the ratio of the observed microscopic rate constants.

Calculated using CCP4. (b) Pair-wise structural comparison (DALI) of APC/C subunits. (c) Surface Plasma Resonance data. Table comparing APC/^CCdh1.Hsl1 (ternary APC/C), dissociation constants for UbcH10 (Ref. 4), and UbcH10^LR (this work). K^eq_d: Equilibrium dissociation constant; K^kin_d: kinetic dissociation constant = k_off/k_on. Standard errors of the fit are listed.