Figure 7.

Quantification of cardiomyocyte apoptosis between WT and GSNOR^−⁄− hearts post MI. A and B, Immunofluorescent images of cleaved caspase-3 (Casp. 3), a marker of apoptotic cell death, in WT (left panel) and GSNOR^−⁄− (right panel) hearts, 1 week post MI. C, Quantification of activated Casp. capsase-3⁺ cardiomyocytes. Compared with baseline, the numbers of apoptotic cardiomyocytes expressing activated caspase-3 are dramatically increased within 1 week post MI. However, no significant differences are detected in apoptotic cardiomyocytes between WT and GSNOR^−⁄− mice. (Kruskall–Wallis, *P=0.0002). Values are mean±SEM. AF indicates autofluorescence; GSNOR, S-nitrosoglutathione reductase; MI, myocardial infarction; WT, wild-type.