Table 3.
Highly over-expressed genes identified in the white isthmus during deposition of the eggshell membranesa
| Gene symbol | Entrez gene name | WI/Ut (fold change) | WI/Ma (fold change) | Secretion | Confirmed by proteomics | ||
|---|---|---|---|---|---|---|---|
| SignalPb | SecretomePc | ESd membranes | ESd matrix | ||||
| COL10A1 | Collagen α-1(X) chain-like | 111.43; 50.21 | 155.42; 94.35 | Yes | - | [7, 17, 18] | [6, 35, 38] |
| R3HCC1 | R3H domain and coiled-coil containing 1 | 79.34 | 136.24 | - | - | - | - |
| CREMP | PREDICTED: similar to spore coat protein SP75, partial | 70.03 | 58.08 | - | Yes | [7, 17, 18] | - |
| AP1G1 | Adaptor-related protein complex 1, gamma 1 subunit | 45.89 | 15.56 | - | - | - | - |
| FBN1 | Fibrillin 1 | 45.89 | 2.53 | - | - | - | - |
| FCGBP | IgGFc-binding protein-like | 22.63; 7.62 | 34.06; 8.82 | Yes | - | - | - |
| MXI1 | MAX interactor 1 | 14.12 | 9.85 | - | - | - | - |
| LIMCH1 | LIM and calponin homology domains-containing protein 1 | 11.63 | 15.45 | - | - | - | - |
| FAM164C | Family with sequence similarity 164, member C | 3.71 | 2.79 | - | Yes | - | - |
| TDRD9 | putative ATP-dependent RNA helicase | 3.36 | 2.68 | - | Yes | - | - |
| HPX | Hemopexin | 2.95 | 1.57 | - | Yes | [7, 17, 18] | [6, 35, 38] |
| TMEM63C | Transmembrane protein 63C | 2.58 | 2.16 | - | - | - | - |
| SORD | Sorbitol dehydrogenase | 2.48 | 2.25 | - | Yes | - | - |
| PHGDH | D-3-phosphoglycerate dehydrogenase | 1.79 | 2.39 | - | Yes | - | - |
| TXN | Thioredoxin | 1.66 | 2.06 | - | - | [7, 17] | [35] |
aHigh over-expression defined as ≥ 2-fold (Log2 Ratio ≥ 1.0) relative to either the magnum or the uterus
bPredicted by SignalP 4.1 software
cPredicted by SecretomeP 2.0 software
dES Eggshell