Table 2.
Mean of Growth inhibition zone diameter (mm) of ethanol extract of Golnar and its fractions (n = 3)
| Extract concentration | 500 mg/ml | 250 mg/ml | 125 mg/ml | 62.5 mg/ml | 31.25 mg/ml | 15.62 mg/ml | 7.8 mg/ml | 3.9 mg/ml | 1.95 mg/ml | |
|---|---|---|---|---|---|---|---|---|---|---|
| Microorganism | ||||||||||
| Staphylococcus aureus ATCC 25923 | M | 32 ± 0 | 30 ± 0.6 | 29 ± 1 | 28 ± 1 | 27 ± 0 | 26 ± 0.6 | 25 ± 0.6 | 24 ± 1 | 23 ± 0 |
| W | 34 ± 0.6 | 32 ± 1 | 31 ± 1 | 30 ± 0.6 | 28 ± 1 | 27 ± 0.6 | 26 ± 1 | 25 ± 0.6 | 24 ± 0.6 | |
| EA | 31 ± 1 | 28 ± 1 | 27 ± 1 | 26 ± 0.6 | 24 ± 1 | 22 ± 0 | 18 ± 0 | 15 ± 1 | 13 ± 0 | |
| C | 19 ± 1 | 16 ± 0 | 14 ± 0.6 | 13 ± 0.6 | - | - | - | - | - | |
| EE | 32 ± 0 | 30 ± 0 | 28 ± 0.6 | 27 ± 1 | 25 ± 1 | 24 ± 1 | 23 ± 1 | 22 ± 0 | 21 ± 0.6 | |
| Bacillus cereus PTCC 1247 | M | 29 ± 0 | 21 ± 0 | 19 ± 1 | 17 ± 1 | 16 ± 1 | 15 ± 0.6 | 14 ± 0.6 | 13 ± 0.6 | 12 ± 0.6 |
| W | 28 ± 1 | 23 ± 1 | 22 ± 0 | 19 ± 0 | 18 ± 0 | 16 ± 0 | 14 ± 0.6 | 13 ± 0.6 | 12 ± 0.6 | |
| EA | 29 ± 0.6 | 23 ± 0.6 | 20 ± 0.6 | 14 ± 1 | 13 ± 1 | 12 ± 0 | 11 ± 0 | 10 ± 0 | - | |
| C | 17 ± 0 | 13 ± 1 | 12 ± 1 | 11 ± 1 | 10 ± 0.6 | - | - | - | - | |
| EE | 28 ± 0.6 | 25 ± 0.6 | 23 ± 0 | 19 ± 0 | 18 ± 1 | 16 ± 1 | 15 ± 1 | 12 ± 0 | 11 ± 0 | |
| Listeria monocytogenes ATCC 7644 | M | 30 ± 0 | 26 ± 1 | 22 ± 0.6 | 19 ± 0.6 | 17 ± 0.6 | 15 ± 0 | 14 ± 0 | 11 ± 0 | 10 ± 0.6 |
| W | 30 ± 0.6 | 25 ± 1 | 23 ± 1 | 20 ± 0 | 17 ± 0 | 15 ± 0 | 14 ± 0.6 | 12 ± 0.6 | 11 ± 0.6 | |
| EA | 28 ± 0 | 22 ± 0 | 20 ± 1 | 17 ± 0.6 | 13 ± 1 | - | - | - | - | |
| C | 18 ± 1 | 14 ± 1 | 11 ± 0.6 | - | - | - | - | - | - | |
| EE | 32 ± 1 | 28 ± 1 | 26 ± 1 | 26 ± 0 | 22 ± 0 | 18 ± 0 | 14 ± 0.6 | 13 ± 0.6 | 11 ± 0.6 | |
| Shigella dysantriae PTCC 1188 | M | 32 ± 0.6 | 29 ± 0.6 | 28 ± 0.6 | 28 ± 0 | 27 ± 0 | 26 ± 1 | 25 ± 1 | 24 ± 0 | 23 ± 0.6 |
| W | 30 ± 1 | 28 ± 0.6 | 27 ± 0.6 | 26 ± 0 | 25 ± 0 | 24 ± 0 | 22 ± 0.6 | 20 ± 1 | 19 ± 1 | |
| EA | 30 ± 0 | 27 ± 0 | 24 ± 0.6 | 21 ± 0.6 | 20 ± 0.6 | 19 ± 0 | 17 ± 0 | 15 ± 0.6 | 12 ± 0.6 | |
| C | 18 ± 0.6 | 15 ± 1 | 13 ± 1 | 12 ± 1 | - | - | - | - | - | |
| EE | 30 ± 1 | 27 ± 0 | 25 ± 0 | 24 ± 0.6 | 23 ± 1 | 21 ± 1 | 20 ± 1 | 19 ± 0 | 18 ± 0 | |
| Salmonella typhi ATCC 19430 | M | 31 ± 0.6 | 24 ± 0 | 21 ± 0 | 18 ± 0.6 | 14 ± 0.6 | 13 ± 0.6 | 12 ± 0.6 | 11 ± 0.6 | 10 ± 0 |
| W | 30 ± 0 | 25 ± 0 | 22 ± 0 | 16 ± 0.6 | 13 ± 0.6 | 12 ± 0.6 | 10 ± 0.6 | - | - | |
| EA | 23 ± 0.6 | 19 ± 1 | 15 ± 1 | 12 ± 1 | 11 ± 1 | - | - | - | - | |
| C | 14 ± 1 | 10 ± 1 | - | - | - | - | - | - | - | |
| EE | 27 ± 0.6 | 24 ± 0 | 22 ± 0 | 21 ± 0 | 17 ± 0 | 14 ± 0 | 11 ± 0.6 | - | - | |
| Escherichia coli ATCC 25922 | M | 28 ± 1 | 19 ± 1 | 15 ± 0.6 | 11 ± 0 | 10 ± 0 | - | - | - | - |
| W | 25 ± 0.6 | 15 ± 0.6 | 12 ± 0 | 10 ± 0 | - | - | - | - | - | |
| EA | 23 ± 1 | 18 ± 1 | 15 ± 1 | 12 ± 0.6 | - | - | - | - | - | |
| C | 12 ± 0 | - | - | - | - | - | - | - | - | |
| EE | 22 ± 1 | 15 ± 0.6 | 13 ± 0.6 | 10 ± 0 | - | - | - | - | - | |
M, W, EA, C, and EE represents methanol, water, ethyl acetate fractions and ethanol extract, respectively, Zone of inhibition, including the diameter of the well (6 mm); mean ± SD value of three independent experiments