Fig. 7.

RT-qPCR validation of RNA-seq results. Five genes were selected for DE confirmation in the same RNA samples used for RNA-seq (G3), a laboratory raised colony from west-Africa (IRSS), and wild-caught mosquitoes from the Kodeni region of Burkina Faso (Kodeni). A: Log₂ fold-change comparison with standard deviation (does not apply to RNA-seq results), B: Correlation analysis between RNA-seq and RT-qPCR log₂ (fold-change) results from the same RNA samples (An. gambiae G3 strain). Spearman correlation coefficient is displayed (r). GSTd3: glutathione-S-transferase, delta class, member 3; ODC: ornithine decarboxylase; ABCA3: ABC transporter, subfamily A, member 3; Tpx: thioredoxin peroxidase; CYP9J5: cytochrome P450, family 9, subfamily J, member 5