Table 3.
Peritrophic matrix-associated transcripts differentially expressed after IVM ingestion
| Gene ID | Description | 2IVM:2BF FC | 6IVM:6BF FC |
|---|---|---|---|
| AGAP000570 | salivary or midgut protein | 37.57 | 18.30 |
| AGAP002643 | No description | 21.93 | 7.95 |
| AGAP006400 | Alkaline phosphatase 2 | 21.74 | 5.85 |
| AGAP001819 | Predicted PM protein, Dinglasan et al. 2009 [97] (AgAper25b) | 20.03 | 12.60 |
| AGAP008648 | No description | 18.65 | 15.74 |
| AGAP002848 | Niemann-Pick Type C-2 | 16.83 | 11.92 |
| AGAP007745 | No description | 15.46 | 7.79 |
| AGAP006795 | Chitin-binding peritrophin (AgAper1) | 12.74 | 6.91 |
| AGAP006432 | ICHIT (AgChitinase) | 9.75 | 3.13 |
| AGAP006414 | chitinase | 7.80 | 4.64 |
| AGAP001199 | serine protease | 7.12 | 5.70 |
| AGAP009830 | predicted PM protein, Dinglasan et al. 2009 [97] (AgAper14) | 6.84 | 11.36 |
| AGAP003713 | No description | 6.23 | 11.06 |
| AGAP010056 | hexosaminidase | 4.84 | 3.32 |
| AGAP006398 | No description | 3.94 | 3.38 |
| AGAP006442 | No description | 2.63 | 4.35 |
| AGAP004918 | fibrinogen | 2.51 | 2.33 |
| AGAP006102 | PRS1 (ribose phosphate diphosphokinase subunit), parital ( An. gambiae ) | 2.45 | 2.90 |
| AGAP001193 | No description | 2.26 | 3.13 |
| AGAP011630 | No description | 2.16 | 2.28 |
| AGAP008487 | sphingomyelin phosphdiesterase ( An. darlingi ) | 2.06 | 5.38 |
| AGAP010733 | Vanin-like protein 1 | 2.05 | 2.20 |
| AGAP004809 | protease m1 zinc metalloprotease | 21.09 | - |
| AGAP011302 | alkaline phosphatase | 12.51 | - |
| AGAP010383 | Solute carrier family 15 member 1 | 9.20 | - |
| AGAP008370 | carboxypeptidase A | 7.91 | - |
| AGAP011305 | alkaline phosphatase | 5.32 | - |
| AGAP011453 | transferrin ( An. darlingi ) | 5.19 | - |
| AGAP004860 | protease m1 zinc metalloprotease | 4.83 | - |
| AGAP006434 | Predicted PM protein, Dinglasan et al. 2009 [ 97 ] (AgAper57) | 4.46 | - |
| AGAP006433 | Predicted PM protein, Dinglasan et al. 2009 [ 97 ] (AgAper34) | 3.34 | - |
| AGAP008861 | female reproductive tract protease GLEANR_896 | 3.08 | - |
| AGAP006709 | Chymotrypsin-1 | −4.23 | - |
| AGAP007142 | Serine-type enodpeptidase | - | 15.35 |
| AGAP005065 | loosely serine-type endopeptidase ( Aedes aegypti ) | - | 13.64 |
| AGAP010383 | Solute carrier family 15 member 1 | - | 10.06 |
| AGAP006385 | protease or trypsin (late or precurser) | - | 9.29 |
| AGAP008292 | Trypsin-4 | - | 3.47 |
| AGAP008294 | Trypsin-3 | - | 2.94 |
| AGAP006796 | peritrophin (Aper9) | - | 2.87 |
| AGAP001198 | chymotrypsin | - | 2.46 |
| AGAP004142 | aspartate aminotransferase, cytoplasmic | - | 2.18 |
| AGAP006416 | Serine protease SP24D | - | 2.14 |
| AGAP004883 | No description | - | 2.04 |
| AGAP008295 | Trypsin-2 | - | −4.17 |
Peritrophic matrix associated transcripts significantly differentially expressed following IVM ingestion. Inclusion based on identification of PM proteins by Dinglasan et al. [97], in which the An. gambiae adult proteome was thoroughly characterized by mass spectrometry, identifying a total of 209 proteins. Except those indicated as Dinglasan et al., descriptions were provided by VectorBase and descriptions in bold data were annotated based on BLASTp results (e-value ≤ 10−3) (Additional file 10)