Table 1.

Chromatographic program with the following mobile phase compositions: mixture of heptane and isopropanol 98:2 (v/v) (phase A); mixture of chloroforme and isopropanol 65:35 (v/v) (phase B); mixture of methanol and water 95:5 (v/v) (phase C); isopropanol (phase D)

Time	A	B	C	D
0	98	2	0	0
2	98	2	0	0
8	12	88	0	0
22	0	60	40	0
26	0	60	40	0
27	0	0	0	100
29	0	0	0	100
30	98	2	0	0
44	98	2	0	0

All A, B and C mobile phases also contain acid acetic 1% (v/v) and triethylamine 0.08% (v/v)