Fig. 3.

Dose and temporal responses of FOXO3-GFP translocation. a MCF7 cells transfected with FOXO3-GFP were serum starved for 24 h and treated with the indicated concentrations of estrogen or G-1 for 15 min. b and c MCF7 cells transfected with FOXO3-GFP, serum starved for 24 h and treated with 0.1 % DMSO, 50 nM estrogen, 100 nM G-1 (b) or 50 ng/ml EGF (c) for 5, 15 or 30 min. Results are reported as mean +/− s.e.m. from at least 3 experiments. *, p < 0.05; **, p < 0.01 vs. DMSO control