Fig. 5.

Estrogen receptor antagonist-stimulated FOXO3-GFP translocation in MCF7 cells requires GPER. MCF7 cells were transfected with either siControl, siGPER or siERα and FOXO3-GFP followed by serum starvion for 24 h prior to treatments as in Fig. 4. Cells were treated with 0.1 % DMSO, 50 nM estrogen, or 1 μM ICI182,780, raloxifene or 4-hydroxytamoxifen (Tamoxifen). Results are reported as mean +/− s.e.m. from at least 3 experiments. *, p < 0.05; **, p < 0.01 vs. DMSO control of the matched siRNA. #, p < 0.05 vs. the ligand-matched siControl treatment