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. 2015 Oct 15;14:165. doi: 10.1186/s12934-015-0356-8

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© Čiplys et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — SDS-PAGE analysis of yeast culture media and purified recombinant human CRT samples. Upper panel yeast culture media after CRT expression in P. pastoris and S. cerevisiae (10× concentrated media supernatants). A media from yeast strains expressing human CRT. B media from control yeast cells without CRT gene. Lower panel purified secreted recombinant human CRT protein (C lanes). M unstained protein ladder (ThermoScientific, cat. no. 26614)