Fig. 3.

Tryptic peptide mass fingerprinting of yeast-secreted CRT protein. Purified secreted CRT protein was analyzed by trypsin digestion and MALDI-TOF/TOF tandem MS/MS (upper panels) or nano-LC coupled with HDMS Synapt G2 mass spectrometer (lower panels). Human CRT was identified with ~66 % or ~91 % sequence coverage in S. cerevisiae- or P. pastoris-derived samples, respectively (identified sequences are indicated in bold). The N-terminal tryptic peptide (begins not from lysine or arginine), and C-terminal tryptic peptide (contains C-terminal -KDEL sequence), are underlined