Fig. 9.

SDS-PAGE analysis of culture supernatant from P. pastoris fermentation during the methanol fed-batch stage. Recombinant human CRT was produced as shown in Fig. 8 and described in the text. 2.5 µl of P. pastoris culture media, taken at different time points, was loaded onto each lane. M prestained protein ladder (ThermoScientific, cat. no. 26616)