Skip to main content
. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Sep 11;1003:12–21. doi: 10.1016/j.jchromb.2015.09.004

Figure 3.

Figure 3

Plasma concentrations of the detected compounds after p.o. administration of XCHT in A/J mice. Blood sample (10 μL) was spiked with 10 μl of 50% methanol, add 2 μL Vitamin C (20%), which was further extracted by 200 μL internal standard solution (0.5 μM daizein in methanol) by vortex-mixing for 1 min. After centrifugation at 20,000 × g for 15 min, the supernatant was transferred to a new tube and evaporated to dryness under a stream of air. The residue was reconstituted in 80 μL of 50% acetonitrile and centrifuged at 20,000 × g for 15 min for LC-MS injection. The circled concentrations were out of the linear range.