Table 3.

Intra-day and inter-day precisions and accuracies for the determination of seventeen compounds from the assay samples (mean±SD, n = 3)

components	C (nM)	Inter-day			Intra-day
		Observed C (nM)	Precise (RSD, %)	Accuracy (Re, %)	Observed C (nM)	Precise (RSD, %)	Accuracy (Re, %)
Liquiritin	0.488	0.571±0.079	13.9	11	0.5±0.027	5. 4	2.5
	62.5	57.93±0.42	4.1	−7.3	62.4±6.44	10.3	−0.1
	500	470.3±34.9	7.4	−5.9	521.7±19.3	3.7	4.3
rutin	0.0488	0.0536±0.006	11.2	13	0.0485±0.004	8.7	−0.5
	6.25	6.29±0.609	9.7	0.6	6.25±0.63	10.1	0
	50	45.6±3.3	7.2	−8.7	49.1±6.9	14.1	−1.8
zingerone	3.9	3.34±0.452	13.47	12.7	3.37±0.46	13.6	−13.5
	62.5	56.03±6.5	11.6	−10.3	62±6.4	10.3	−0.8
	500	489±12.4	12.4	−2.2	522.33±56.72	10.9	4.5
baicalin	3.9	0.369±0.0313	8.46	8.46	3.87±0.47	12.1	−0.8
	62.5	1.793±0.298	16.6	16.6	54.5±4.9	8.9	−12.8
	500	52.56±1.401	2.67	2.67	513±67	13.1	2.6
quercetin	3.9	4.13±0.298	7.22	7.22	4.36±0.395	9.1	11.7
	62.5	60.2±7.3	12.2	−3.6	60.4±7.4	12.3	−3.3
	500	438.7±21.22	4.8	−12.3	470±44.9	9.6	−6
Rg1	0.488	0.466±0.065	13.9	−4.5	0.448±0.017	3.7	−8.2
	62.5	59.13±7.22	12.2	−5.4	71.7±5.76	8	14.8
	500	468±22.91	4.9	−6.4	474±13.5	2.9	−5.2
Re	3.9	3.63±0.467	12.9	−6.9	3.52±0.274	7.8	−9.7
	62.5	66.6±3.86	5.8	6.6	68.7±3.1	4.5	9.9
	500	497.33±6.65	1.3	−0.5	472±27.1	5.7	−5.6
wogonoside	0.488	0.463±0.03	6.5	−5.1	0.469±0.001	0.86	−4
	62.5	58.7±4.1	7	−4.1	63.8±0.69	1.1	2.1
	500	472.3±28.3	6	−5.5	488±41.7	8.5	−2.4
baicalein	3.9	3.84±0.344	8.95	−0.03	3.90±0.21	5.5	0.2
	62.5	62.1±7.1	11.4	−0.6	64.97±4.5	7	3.9
	500	423.3±20.5	4.8	−15.3	453.3±11.84	2.6	−9.3
Formononetin	3.9	4.1±0.28	4.8	5.1	3.47±0.129	3.7	−10.9
	62.5	63.56±2.9	4.6	1.7	72.4±6.05	8.3	15.9
	500	491±13.2	2.7	−1.8	475.3±26.9	5.7	−4.9
wogonin	0.488	0.461±0.014	3	−6.5	0.421±0.0456	10.9	−13.7
	62.5	57.3±3.38	5.9	−8.4	62.9±3.91	6.2	0.6
	500	471±36.3	7.7	−5.8	486.3±23.5	4.8	−2.7
(6)-Gingerol	3.9	3.93±0.19	4.83	0	4.08±0.145	3.6	4.7
	62.5	54.06±6.38	11.8	−12.6	65.6±6.4	9.7	5
	500	443.7±67.7	15.3	−11.3	514.3±33.5	6.5	2.9
SSa	0.39	0.367±0.05	13.8	−5.8	0.043±0.006	12.1	0.7
	6.25	5.84±0.517	8.9	−6.5	6.04±0.262	4.3	−3.4
	50	48.96±6.14	12.5	−2.1	52.6±1.97	3.7	5.2
Rd	0.488	0.504±0.071	14.16	3.3	0.479±0.047	9.9	−1.7
	62.5	63.5±7.0	11	1.6	60.86±3.9	6.5	−2.6
	500	527±33.45	6.3	5.4	477.7±23.54	4.9	−4.5
SSd	0.0488	0.050±0.006	12	1	0.0398±0.0	0	−18
	6.25	6.16±0.44	7.2	−1.4	6.59±0.113	1.7	5.4
	50	49±3.16	6.4	−1.9	50.16±2.35	4.7	0.3
Rg3	0.488	0.482±0.074	15.1	−1.1	0.466±0.0056	1.2	−4.5
	62.5	59.7±1.31	2.2	−4.5	63.5±2.54	4	1.7
	500	461.7±36.5	7.9	−7.7	494.3±27.31	5.5	−1.1
GA	0.488	0.462±0.038	10.8	−5.4	0.489±0.0356	7.3	0.3
	62.5	58.7±6.17	10.5	−6.1	63.46±5.61	8.8	1.5
	500	485.3±33	6.8	−2.9	529.33±25	4.7	5.9