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. 2015 Oct 13;6:8533. doi: 10.1038/ncomms9533

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Vaccination with MtbΔsigH (dark blue) induced a significantly stronger T_H1 cell response relative to BCG vaccination (light blue), or no vaccination (red). Quantification of and representative histograms of CD4⁺CD69⁺ (a) and CD8⁺CD69⁺ (b) T cells migrating to the lung after vaccination. Absolute cell counts of phenotypic markers CXCR3, CCR5 and CXCR4 in CD4⁺ (c) and CD8⁺ (d) T cells in BAL at different stages of infection. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 using two-way ANOVA with Tukey's correction for multiple comparisons. Data are means±s.d. BAL samples from all 21 animals (n=7, three groups) were included in the flow cytometry experiments and analyses. Samples from all seven animals in each group were used for analysis at each time point.