Table 4.

Primers and PCR products used in this study

Primer or PCR product	Nucleotide sequence (5′–3′) or description	Amplification
Primers
JJ3-F	ACCACTCATAGTGGAAAATATTATGG	AI
JJ5-R	GGCGGAGCTCTCCAGGCATTAAAATTGAGATTTATCTCCATAATC	AI, UA, UAI
USPNC-F	GAATTCTCACCATGGGAAAAAAAAAGATTATCTCAGCTATTTTAATGTCTAC	UA, UAI
JJ8-R	CCATAATATTTTCCACTATGAGTGGTAGCGTAAACACCTGACAACGG	UA
PCR products
AI	475-bp XhoI fragment containing the mature enterocin A (entA) and immunity (entiA) genes
UA	124-pb NcoI fragment containing the usp45 signal peptide (SPusp45) and the begining of mature entA
UAI	567-bp NcoI/XhoI fragment containing the SPusp45 fused to mature entA and entiA