FIGURE 3.

Structure of the high-affinity phosphate transporter, PiPT. The structure represents an inward facing occluded state of the phosphate transporter in complex with phosphate. (A) Phosphate (shown as spheres) is buried in the membrane at the interface between the N domain (pale green) and C domain (blue). Selected residues are shown as sticks. Black bars depict the approximate location of the membrane. (B) The phosphate-binding site with yellow dashes indicating possible hydrogen bonds (2.2–3.8Å distances) to phosphate. Top: the omit 2mFo-DFc density for phosphate is contoured in orange (4s). Bottom: the 2mFo-DFc density for phosphate and selected M7 residues is contoured in red (2s). Reproduced with permission from Macmillan Scientific Publishers Ltd. (London). For more detailed information on PiPT structure please see reference Pedersen et al. (2013).