Table 1.
Strain and plasmid list.
| Strain or plasmid | Relevant genotype | Reference |
|---|---|---|
| Strains | ||
| Staphylococcus aureus | ||
| Newman D2C | wild-type SrtA positive; non-hemolytic; coagulase negative | ATCC25904 |
| ΔSrtA | srtA::Erm; isogenic mutant of Newman D2C | Chen et al., 2013 |
| Escherichia coli | ||
| BL21 | Expression strain, F- ompT hsdS(rB- mB-) gal dcm (DE3) | Invitrogen |
| Plasmids | ||
| pGEX-6P-1 | Expression vector | Amersham |
| pGSrtAΔN59 | pGEX-6P-1 with srtA gene | This study |
| C184A | pGSrtAΔN59 derivative, for the substitution of Cys184 with alanine | This study |
| G192A | pGSrtAΔN59 derivative, for the substitution of Gly192 with alanine | This study |