Table 2.

Oligonucleotide primers used in this study.

Primer name	Oligonucleotide (5–3)a
PsrtA59F	GCGGGATCCCCGGAATTCCAAGCTAAACCTCAAATTCC
PsrtA59R	CCGCTCGAGTTATTTGACTTCTGTAGCTACAA
C184A–F	GCCTGTCTTTTCATTGTAATCATCAGCAGTAATTAATGTT
C184A–R	GCTGATGATTACAATGAAAAGACAGGCGTTTGGGAAAAAC
G192A–F	TATTTAATTGTTCAGCTGTTGCTGGT
G192A–R	GCTGAACAATTAAATAGAGGTGTAA

^a Restriction endonuclease recognition sites or mutated codons are underlined.