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The calculated energy components, binding free energy (kcal/mol), binding constants, and the number of binding sites using the fluorescence spectroscopy quenching method in the WT-CHA, C184A-CHA, and G192A-CHA systems.
| TΔS (kcal/mol) | ΔGbind (kcal/mol) | Binding constants KA (1 × 105) L⋅mol-1 | n | |
|---|---|---|---|---|
| WT-CHA | 6.7 ± 2.0 | -15.2 ± 2.4 | 8.9 ± 1.3 | 0.9987 |
| C184A-CHA | 5.9 ± 1.4 | -7.9 ± 1.6 | 4.7 ± 0.8 | 1.0025 |
| G192A-CHA | 6.3 ± 1.3 | -8.4 ± 1.9 | 5.0 ± 0.9 | 0.9995 |
