Table 2.

Primers Used for q-RT PCR

Gene:	Primer Sequences:
PKG1	Forward: 5′ ATG CAA AGA CTG GCC AAG CTA C 3′
	Reverse: 5′ AGC CAC AGC CTC AGC ATA TTT C 3′
HO-1	Forward: 5′ TGT AAG GGA GAA TCT TGC CTG GCT 3′
	Reverse: 5′ TGC TGG TTT CAA AGT TCA GGC CAC 3′
NQO1	Forward: 5′ AAG AGC TTT AGG GTC GTC TTG GCA 3′
	Reverse: 5′ AGC CTC CTT CAT GGC GTA GTT GAA 3′
XBP-1 Spliced	Forward: 5′ GAG TCC GCA GCA GGT G 3′
	Reverse: 5′ GCT TAG AGG TGC TTC CTC AAT 3′
XBP-1 Total	Forward: 5′ CAC CTC TGC AGC AGG TG 3′
	Reverse: 5′ GCT TAG AGG TGC TTC CTC AAT 3′
BiP	Forward: 5′ TGG ATA AGA GAG AGG GAG AGA AG 3′
	Reverse: 5′ GTG AGA AGA GAC ACA TCG AAG G 3′
CHOP	Forward: 5′ CAG CGA CAG AGC CAG AAT AA 3′
	Reverse: 5′ CAG GTG TGG TGG TGT ATG AA 3′
Ire1α	Forward: 5′ TCC TAA CAA CCT GCC CAA AC 3′
	Reverse: 5′ TCT CCT CCA CAT CCT GAG ATA C 3′
ATF6	Forward: 5′ CTC GGC TCG GTA GTT TGT ATC 3′
	Reverse: 5′ AGA CCT GAA TGG CTG CTT AC 3′
ATF4	Forward: 5′ CCA CTC CAG AGC ATT CCT TTA G 3′
	Reverse: 5′ CTC CTT TAC ACA TGG AGG GAT TAG 3′

Primer pairs used for qRT-PCR obtained from Integrated DNA Technologies (Coralville, IA) are shown.

Abbreviations are: PKG1, Protein Kinase Gene 1; HO-1, Heme Oxygenase-1; NQO1, NAD(P)H:quinone oxidoreductase; XBP-1, X-box Binding Protein 1; BiP, Binding Immunoglobulin Protein; CHOP, C/EBP homologous protein; Ire1α, inositol-requiring enzyme 1 alpha; ATF6, activating transcription factor 6; ATF4, activating transcription factor 4.