Table 3.

mRNA alterations in Endogenous Antioxidant Response Genes Following Antiretroviral Application in Primary Oligodendrocyte Precursor Cells

Gene	Treatment	ΔΔCT ± SE	Fold Change Relative to Control	P value
HO-1	AZT	−0.639 ± 1.356	1.558 (0.609–3.987)	0.3503
	Ritonavir	1.116 ± 1.363	0.461 (0.179–1.187)	0.9402
	Lopinavir	0.6117 ± 0.9833	0.654 (0.331–1.294)	<0.0001 *
NQO1	AZT	−0.14 ± 2.714	1.102 (0.168–7.230)	0.7153
	Ritonavir	−0.780 ± 0.221	1.716 (1.473–2.00)	0.015 *
	Lopinavir	−0.195 ± 1.2	1.272 (0.554–2.921)	<0.0001 *

Extracted RNA (5 μg) was converted to cDNA using the Superscript First-strand kit and q-PCR was performed using Power SYBR Green. All measurements were normalized first to the oligodendrocyte housekeeping gene PKG1, and then to controls. Analysis was performed by the ΔΔCT method. Endogenous Antioxidant Response genes: HO-1 and NQO1 were probed. ΔΔCT ± SE, fold change, and fold change range are presented from 3 independent biological replicates (n = 3). P values are indicated;

^*p < 0.05. AZT, Zidovudine.