Figure 1.

Identification of neutrophils stained by antibodies against CD11c, CD64 and epidermal growth factor (EGF)-like molecule containing mucin-like hormone receptor (EMR2). Flow cytometric analysis identified neutrophils in whole blood samples according to (a) their characteristic granularity (side-scatter), nuclear staining (positive for LDS-751) and (b) staining with fluorescein isothiocyanate (FITC)-conjugated monoclonal antibody against the neutrophil marker CD66b. Profiles of neutrophils from patients with systemic inflammatory response syndrome (SIRS) (shaded histograms) and healthy control subjects (open histograms) stained with antibodies against (c) CD11c, (d) CD64 and (e) EMR2 (dark lines) and isotype control antibodies (light lines).