Fig 9. Alignment of 71cR amino acid sequence with the 130 amino acid sequences in S1 Table.

Chimera 1.10.1 was used to display the degree of conservation for each residue within the alignment of the conserved domain [38]. 71cR C. nicotianae sequence is shown along with the consensus sequence from the whole dataset. The numbers above the amino acid residues indicate the positions within the sequence alignment. Color of each amino acid was determined according to the ClustalX [42] color scheme. The bars show the conservation between 71cR and all of the 130 sequences in S1 Table. Stars on top of the amino acids indicate the complete conservation of that sequence except the outgroup (E on position 135 is completely conserved throughout every single one including the outgroup). The red boxes around the letters show the ligand binding sites predicted by Raptor software.