Table 1. Primer sets used for RT-qPCR analysis for screening C. nicotianae hypothetical protein genes for induction under cercosporin toxicity conditions.

Gene	Forward Primer	Reverse Primer	Efficiency 1
11sf	AGTGGCTAATCTGCTCTGG	CACTGCTATTCCTGTTGG	100.08
15cF	ACATGCCGCACTCTGTTCATTTGG	GAAGCCCACAACCTGCAACCATTT	104.48
1cF	CCGTTCCTGCAGAGCTCAAG	GCCATTGACCATGTTGAGCATGTC	109.33
200sF	TGCTGATCCGCTAAATGCTAAAAC	GCCGATGGACAAGGGTATAAGATC	100.21
207sF	ATGCTCGATCCTCCGAACCA	CGGCAGCTTTGAGCGTCTTT	101.78
214sR3	ATCAGAAGAGACAGCATAAAGC	AAGTGGTGGCTCAGCGTGG	89.57
24cF	TGCTTTCACCTTCAAGTTCGAC	TGCCCTTGCCAAAGCTAGG	106.51
40cR	TTGGTCCATCCCTGATCCTGTTGT	TCAGACTCAGCGAGCGAAGGATTT	99.25
55cR	GGAGACAGCCAAGCAAGAAGTC	GGGAAGAAGGCGATTGAGGA	100.16
56cR	ATCGTTCAAGACCGAGAGGCTCAA	ATGCCGAGAGATCAATGTCCCGAT	105.38
71cR	TCAAGCCACCCTACAATGCCTCAA	TTATTTGGTCGGTGCCTTGGACGA	100.09
77sR3	TGAGTGGTCGCTTGATTCG	ATCTGGACCCGAAATCGTGC	97.62
84sF	AGTGGGAGTGGGAGTCTTGG	AACACTGGAGAACGAATCAACG	91.29
Actin	TGACGATGCGCCACGAGCTGT	TTGATTGGAGCCTCGGTGAGC	101.20

¹ Calculated efficiencies for primers for RT-qPCR analysis