Figure 3.

The n-butanol extract from Folium isatidis inhibited LPS-induced MyD88 recruitment, IκB-α degradation, and ERK phosphorylation.

Notes: Following pretreatment with vehicle control (DMSO) or a different concentration of the n-butanol extract for 2 hours, MPMs were incubated with LPS (0.5 µg/mL) for 30 minutes. The protein levels of MyD88 (A) and IκB-α (B) were examined using western blotting and were normalized to GAPDH. The protein levels of p-ERK, p-p38, and p-JNK (C) were examined using western blotting and were normalized to ERK, p38, and JNK. Statistical significance compared with the LPS group is indicated, *P<0.05, **P<0.01.

Abbreviations: ERK, extracellular signal-regulated kinase; JNK, c-jun N-terminal kinase; LPS, lipopolysaccharide; MPM, mouse peritoneal macrophage.