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. 2015 Oct 16;10(10):e0140452. doi: 10.1371/journal.pone.0140452

Fig 2. AMPK inhibited IL-6 and NO production in BMDMs stimulated with TLR2 and TLR4 agonists.

Fig 2

(A,B) BMDMs were pre-treated with A-769662 (100, 250 or 500 μM) for 2 hours before stimulated with LPS (1 μg/ml) and Pam3Cys4 (1 μg/ml) for 18 hours. The conditioned media was subjected to ELISA and Griess reaction for the indicated cytokine and NO release, respectively. (C,D) BMDMs were pre-treated with A-769662 (100 μM) for 2 hours before stimulated with LPS for two hours. RNA was isolated and analyzed for the expression of the indicated genes. (E,F) BMDMs were pre-treated with A-769662 (100 μM) for 2 hours before stimulated with LPS (1 μg/ml) and Pam3Cys4 (1 μg/ml) for 18 hours. The conditioned media was subjected to ELISA for the indicated cytokines. Results are expressed as means ± SEM.* p< 0.05 vs DMSO; ** p<0.01; *** p<0.001 and are representative of four independent experiments.