Skip to main content
. 2015 Oct 16;10(10):e0140452. doi: 10.1371/journal.pone.0140452

Fig 3. A-769662 treatment activated AMPK in joints and diminished clinical arthritis in passive K/BxN arthritis.

Fig 3

WT mice were injected with 150μl serum from adult K/BxN mice on day 0 to induce arthritis. A-769662 was administrated twice a day from day 0. (A) Proteins of joint tissues from naive and arthritic mice injected with control vehicle (DMSO) and A-769662 (60mg/kg) were extracted and analyzed for phospho-AMPKα, phosphor-ACC, total AMPKα and total ACC by WB. D as DMSO and A as A-796662. Semi-quantitative densitometry analysis of Western blots (arbitrary densitometry units) for phosphorylation of AMPKα and ACC normalized to total AMPKα and ACC respectively is shown. (B) Clinical score at day 7 in mice treated with vehicle, A-769662 at high (60mg/kg) and low dose (30mg/kg) (n = 5 mice per group). (C) Ankle thickness in DMSO-treated animals (black circles, n = 9) and high dose A-769662-treated animals (black squares, n = 5) injected with 150 μl of K/BxN serum on day 0. Values are means ± SEM. * = p<0.05; ** = p<0.01.