Table 2. MiRNA whose expression was affected by food deprivation in lactating goat mammary gland.
| miRNA name | Family | Sequence | Control* | FD* | Fold change (FD/control) | P-value adjusted |
|---|---|---|---|---|---|---|
| Known miRNA | ||||||
| miR-6119-5p | AGAGGTAAAAAATTGATTTGACT | 19,082 | 14,396 | 0.77 | 0.07 | |
| miR-126-3p | mir-126 | TCGTACCGTGAGTAATAATGC | 17,218 | 22,489 | 1.28 | 0.06 |
| miR-660-5p | mir-188 | TACCCATTGCATATCGGAGCTGT | 7,698 | 6,144 | 0.81 | 0.09 |
| miR-99a-5p | mir-10 | AACCCGTAGATCCGATCTTGT | 5,533 | 7,709 | 1.35 | 0.06 |
| miR-451-5p | mir-451 | AAACCGTTACCATTACTGA | 4,957 | 6,855 | 1.33 | 0.1 |
| let-7c-5p | let-7 | TGAGGTAGTAGGTTGTATGGTT | 4,934 | 6,427 | 1.28 | 0.06 |
| miR-140-3p | mir-140 | ACCACAGGGTAGAACCACGGAC | 3,798 | 4,888 | 1.27 | 0.09 |
| miR-409-3p | mir-154 | GAATGTTGCTCGGTGAACCCC | 1,954 | 1,159 | 0.66 | 0.06 |
| miR-125b-3p | mir-10 | ACAAGTCAGGCTCTTGGGACC | 1,918 | 2,496 | 1.27 | 0.06 |
| miR-222-3p | mir-221 | AGCTACATCTGGCTACTGGGTCTC | 812 | 1,408 | 1.47 | 0.09 |
| miR-204-5p | mir-204 | TTCCCTTTGTCATCCTATGCCT | 541 | 1,113 | 1.8 | 0.00 |
| miR-99a-3p | mir-10 | CAAGCTCGCTTCTATGGGTCTGT | 472 | 615 | 1.27 | 0.08 |
| miR-196a-5p | mir-196 | TAGGTAGTTTCATGTTGTTGG | 413 | 663 | 1.47 | 0.06 |
| miR-494-3p | mir-154 | TGAAACATACACGGGAAACCTCT | 311 | 197 | 0.67 | 0.05 |
| miR-223-3p | mir-223 | TGTCAGTTTGTCAAATACCCCA | 245 | 552 | 1.65 | 0.06 |
| miR-188-5p | mir-188 | CATCCCTTGCATGGTGGAGGG | 117 | 81 | 0.73 | 0.09 |
| miR-671-5p | mir-671 | AGGAAGCCCTGGAGGGGCTGGAGG | 73 | 50 | 0.73 | 0.09 |
| miR-541-5p | mir-541 | AAAGGATTCTGCTGTCGGTCCCACT | 29 | 15 | 0.64 | 0.08 |
| miR-223-5p | mir-223 | TGTGTATTTGACAAGCTGAGTTG | 17 | 34 | 1.6 | 0.06 |
| Predicted miRNA | ||||||
| chr12_17655-3p | CCCGGGTTTCGGCACCA | 611 | 337 | 0.66 | 0.09 | |
| chr19_26739-5p | GAGGGTTTGGGTTTGGTCGTGGGA | 508 | 289 | 0.67 | 0.09 | |
| chr3_4386-5p | ATAGTTCATTCAGGTTTTTCTG | 420 | 227 | 0.66 | 0.09 | |
| chr27_34538-5p | AAAGTTCATTCAGGTTTTTCTG | 412 | 224 | 0.66 | 0.09 | |
| chr3_4386-3p | GAAGAATCTGAATGAACATTT | 253 | 152 | 0.66 | 0.05 | |
| chr23_30758-5p | GGGGATGTAGCTCAGTGGTAGA | 133 | 68 | 0.66 | 0.09 | |
| chr9_13534-5p | GTACATGATGACAACTGGCTC | 83 | 113 | 1.33 | 0.06 | |
| chr12_18027-5p | GAAAGGTTCATTTGGGTTTTT | 56 | 97 | 1.51 | 0.08 | |
| chr22_29775-3p | ATCAGAGTCACGGCACCA | 37 | 16 | 0.59 | 0.05 | |
| chr3_3319-5p | GAAAGTTTGTTTGGGTTTTTC | 39 | 13 | 0.63 | 0.08 | |
| chr4_6064-5p | CCCGATCTTGTCTGATC | 29 | 10 | 0.63 | 0.08 | |
*Mean of normalized read counts.
The seed region of each miRNA is underlined. A positive log2 fold change means the corresponding miRNA is more highly expressed in FD goats than in Controls and vice versa. P-values were adjusted using the Benjamini-Hochberg [35] correction at 10%.