Table 2.
Number of total and viable cells recovered per mg of tissue
| Total cells per mg of tissue (% recovery) | Viable cells per mg of tissue (% recovery) | |||||
|---|---|---|---|---|---|---|
| Fresh cells | Cryo Cells | Cryo Tissues | Fresh Cells | Cryo Cells | Cryo Tissues | |
| Adult Subjects | ||||||
| SID2 | 124,740 | 79,002 (63%) | 26,791 (22%) | 119,751 (96%) | 51,351 (43%) | 20,768 (17%) |
| SID33 | 70,175 | 48,246 (69%) | 27,778 (40%) | 65,965 (94%) | 32,807 (50%) | 21,944 (33%) |
| SID105 | 54,276 | 13,021 (24%) | 10,284 (19%) | 52,105 (96%) | 8,463 (16%) | 7,919 (15%) |
| SID123 | 61,607 | 18,157 (29%) | 9,211 (15%) | 54,214 (88%) | 11,802 (22%) | 7,092 (13%) |
| Ave ± SD | 77,699 ± 32,026 | 39,607a ± 30,515 (46%) | 18,516a ± 10,142 (24%) | 73,009 ± 31,752 | 26,106b ±19,984(33%) | 14,431b ± 8,018(20%) |
| Fetal Testes | ||||||
| Sample 1 | 48,066 | 20,580 (43%) | 33,646 (70%) | 42,586 (89%) | 16,258 (38%) | 28,263 (66%) |
| Sample 2 | 40,441 | 19,485 (48%) | 21,547 (53%) | 36,599 (91%) | 13,834 (38%) | 17,669 (48%) |
| Sample 3 | 40,952 | 17,778 (43%) | 29,549 (72%) | 37,840 (92%) | 13,333 (35%) | 24,230 (64%) |
| Ave ± SD | 43,153 ± 4,262 | 19,281c ± 1,412 (45%) | 28,247c ± 6,154 (65%) | 39,008 ± 3,160 | 14,475d ± 1,564 (37%) | 23,387d ± 5,347 (59%) |
All tissues were weighed prior to processing into single cells or cryopreserved. The number of cells recovered after single cell or tissue cryopreservation was normalized to the original tissue weight in mg prior to processing. Percent total cell recovery was calculated by dividing the total cell number /mg from each experimental group by the total cell number/mg from its freshly digested tissues × 100. Percent viable cell recovery was calculated in similar fashion but with viability assessment as described in Table 1.
p<0.05 compared to total adult and fetal fresh testicular cells, respectively,
p<0.05 compared to viable adult and fetal fresh testicular cells, respectively, by ANOVA with Holm-Bonferroni correction. No significant differences in the recovery rates between cryo cell and cryo tissue subgroups in both adult and fetal samples.