Table 3.
Frequency and number of SSEA-4+ cells per milligram of tissue.
| Frequency of SSEA-4+ Cells | Frequency of Somatic Cells | SSEA-4+ Cells per mg | Somatic Cells per mg | |||||
|---|---|---|---|---|---|---|---|---|
| Cryo Cells | Cryo Tissues | Cryo Cells | Cryo Tissues | Cryo Cells | Cryo Tissues | Cryo Cells | Cryo Tissues | |
| Adult Subjects | ||||||||
| SID2 | 15% | 7% | 16% | 6% | 7,703 | 1,454 | 8,216 | 1,246 |
| SID33 | 30% | 28% | 9% | 5% | 9,842 | 6,144 | 2,953 | 1,097 |
| SID105 | 11% | 7% | 10% | 9% | 931 | 554 | 846 | 713 |
| SID123 | 18% | 11% | 9% | 8% | 2,124 | 780 | 1,062 | 567 |
| Ave ± SD | 19%a ± 8% | 13%a ± 10% | 11% ± 3% | 7% ± 2% | 5,150 ± 4,301 | 2,233 ± 2,635 | 3,269 ± 3,431 | 906 ± 318 |
| Fetal Testes | ||||||||
| Subject 1 | 1.2% | 1.6% | 195 | 452 | ||||
| Subject 2 | 2.5% | 1.3% | 346 | 230 | ||||
| Subject 3 | 1.2% | 1.0% | 160 | 242 | ||||
| Ave ± SD | 1.6% ± 0.8% | 1.3% ± 0.3% | 234 ± 99 | 308 ± 125 | ||||
Frequency of live SSEA-4+ and somatic cells were determined by FACS after excluding dead cells (Figure 1). Cells per mg was calculated by multiplying the frequency with the number of live cells obtained after processing divided by the original weight prior to cryopreservation.
a
p<0.03 by paired student t test.