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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Ann Allergy Asthma Immunol. 2010 Mar;104(3):236–246. doi: 10.1016/j.anai.2009.12.006

Figure 1.

Figure 1

Mice (aged 6 weeks and 6, 12, and 18 months) were sensitized intraperitoneally (i.p) with ovalbumin (OVA) (200 μg per mouse) twice at weekly intervals and then were challenged intratracheally (i.t.) on days 18, 25, and 32 with OVA (100 μg per mouse). One set of mice from each age group was pretreated with intragastric (i.g.) antiasthma simplified herbal medicine intervention (ASHMI) (10 mg/d per mouse) in a twice-daily divided dose starting 4 weeks before sensitization. Treatment was continued through the sensitization and challenge periods. Seventy-two hours after the final OVA challenge, the mice were analyzed. Age-matched naive mice served as additional controls.